-
Product Name
NUMB antibody
- Documents
-
Description
NUMB Mouse Monoclonal antibody. Positive IF detected in A431 cells. Positive WB detected in Human brain. Observed molecular weight by Western-blot: 66-72 kDa
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
h Numb antibody; NUMB antibody; numb homolog (Drosophila) antibody; Protein numb homolog antibody; Protein S171 antibody; S171 antibody
-
Isotype
Mouse IgG2a
-
Preparation
This antibody was obtained by immunization of peptide (Accession Number: NM_001005745). Purification method: Caprylic acid/ammonium sulfate precipitation.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:20-1:200
-
Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107342(NUMB antibody) at dilution of 1:300
Immunofluorescent analysis of A431 cells using Catalog No:107342(NUMB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
-
Background
NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can’t recognize the NUMBL.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"