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NUFIP1 antibody

Cat.#: 113366

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Product Information

  • Product Name
    NUFIP1 antibody
  • Documents
  • Description
    NUFIP1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in A375 cells, HeLa cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 70-75 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    bA540M5.1 antibody; NUFIP antibody; NUFIP1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NUFIP1 recombinant protein (Accession Number: BC017745). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:113366(NUFIP1 antibody) at dilution of 1:400

    A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:113366(NUFIP1 antibody) at dilution of 1:400

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113366(NUFIP1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113366(NUFIP1 antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-NUFIP1 (IP:Catalog No:113366, 4ug; Detection:Catalog No:113366 1:800) with HeLa cells lysate 1200ug.

    IP Result of anti-NUFIP1 (IP:Catalog No:113366, 4ug; Detection:Catalog No:113366 1:800) with HeLa cells lysate 1200ug.

  • Background
    Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of FMRP (Fragile X Mental Retardation Protein). FMRP is an RNA binding protein reported to be involved in translational control, notably at postsynaptic sites of protein synthesis as a part of a multiprotein/mRNA complex[PMID:12941608]. NUFIP1 is one of the several FMRP-interacting proteins. NUFIP can act as a pol II-specific basal transcriptional activator in vitro and when ectopically overexpressed in vivo. NUFIP can directly activate promoters by enhancing the ATP-dependent release of hyperphosphorylated form of pol II from open transcription complexes[PMID:15107825].
  • References
    • Rothé B, Saliou JM, Quinternet M. Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction. Nucleic acids research. 42(16):10731-47. 2014.
    • Bizarro J, Charron C, Boulon S. Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control. The Journal of cell biology. 207(4):463-80. 2014.
    • Bizarro J, Dodré M, Huttin A. NUFIP and the HSP90/R2TP chaperone bind the SMN complex and facilitate assembly of U4-specific proteins. Nucleic acids research. 43(18):8973-89. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"