-
Product Name
NUF2 antibody
- Documents
-
Description
NUF2 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, human liver tissue, human skeletal muscle tissue, human testis tissue, PC-3 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 50kd
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CDCA1 antibody; hNuf2 antibody; hNuf2R antibody; hsNuf2 antibody; KinetOChore protein Nuf2 antibody; NUF2 antibody; NUF2R antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of NUF2 recombinant protein (Accession Number: XM_024450112). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113365(NUF2 antibody) at dilution of 1:500
IP Result of anti-NUF2 (IP:Catalog No:113365, 3ug; Detection:Catalog No:113365 1:500) with HEK-293 cells lysate 1760ug.
-
Background
NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. NUF2 was reported to be overexpressed in various kinds of carcinomas including lung cancer, cholangiocellular cancer, urinary bladder cancer, renal cell cancer, colorectal cancer, gastric cancer, ovarian cancer and serous adenocarcinoma. Elevated expression of NUF2 was associated with poorer prognosis of non-small cell lung cancer patients (PMID: 17079454). Knockdown of NUF2 significantly caused cell growth delay and increased apoptosis in cell lines of non-small cell lung cancer, colorectal cancer, gastric cancer and ovarian cancer.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"