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Product Name
NUDT6 antibody
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Description
NUDT6 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue. Observed molecular weight by Western-blot: 72 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ASFGF2 antibody; FGF 2 antibody; FGF AS antibody; FGF2AS antibody; gfg antibody; gfg 1 antibody; Nudix motif 6 antibody; NUDT6 antibody; Protein GFG antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NUDT6 recombinant protein (Accession Number: NM_007083). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113362(NUDT6 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113362(NUDT6 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-NUDT6 (IP:Catalog No:113362, 4ug; Detection:Catalog No:113362 1:500) with HepG2 cells lysate 4000ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:113362(NUDT6 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.
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References
- Baguma-Nibasheka M, Macfarlane LA, Murphy PR. Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA. Genes. 3(3):505-20. 2012.
- Yuan L, Minghua C, Feifei D. Study of the use of recombinant human granulocyte-macrophage colony-stimulating factor hydrogel externally to treat residual wounds of extensive deep partial-thickness burn. Burns : journal of the International Society for Burn Injuries. 41(5):1086-91. 2015.
- MacFarlane LA, Gu Y, Casson AG, Murphy PR. Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2. Molecular endocrinology (Baltimore, Md.). 24(4):800-12. 2010.
- MacFarlane LA, Murphy PR. Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression. Molecular carcinogenesis. 49(12):1031-44. 2010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"