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Product Name
NUDT16 antibody
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Description
NUDT16 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive IHC detected in human kidney tissue. Positive WB detected in HeLa cells, HEK-293 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ31265 antibody; Nudix motif 16 antibody; NUDT16 antibody; U8 snoRNA binding protein H29K antibody; U8 snoRNA decapping enzyme antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NUDT16 recombinant protein (Accession Number: NM_152395). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113357(NUDT16 antibody) at dilution of 1:2500
IP Result of anti-NUDT16 (IP:Catalog No:113357, 3ug; Detection:Catalog No:113357 1:1000) with HEK-293 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:113357(NUDT16 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:113357(NUDT16 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:113357(NUDT16 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NUDT16 represents nudix (nucleoside diphosphate linked moiety X)-type motif 16
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References
- Shukla S, Parker R. Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion. Proceedings of the National Academy of Sciences of the United States of America. 111(32):E3277-86. 2014.
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