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Product Name
NUDC antibody
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Description
NUDC Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive IF detected in HeLa cells, HEK-293 cells, HepG2 cells. Positive WB detected in HepG2 cells, HEK-293 cells, HeLa cells, K-562 cells, SH-SY5Y cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 42-45 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
HNUDC antibody; MNUDC antibody; Nuclear migration protein nudC antibody; NUDC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NUDC recombinant protein (Accession Number: NM_006600). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113411(NUDC antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113411(NUDC antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-NUDC (IP:Catalog No:113411, 4ug; Detection:Catalog No:113411 1:1000) with HEK-293 cells lysate 2800ug.
Immunofluorescent analysis of HeLa cells using Catalog No:113411(NUDC Antibody) at dilution of 1:25 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Nuclear distribution protein C (NudC) is a highly conserved dynein/dynactin associated protein implicated in mitosis and cytokinesis. NudC is localized on major mitotic structures, and is involved in regulating spindle formation stability of kinetochore microtubule attachments, and chromosomes congression in early mitosis. NudC also is required for multi-protein transport along axons in neurons.
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References
- Zhang C, Zhang W, Lu Y. NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1. Cell research. 26(2):239-53. 2016.
- Kim TY, Siesser PF, Rossman KL. Substrate trapping proteomics reveals targets of the βTrCP2/FBXW11 ubiquitin ligase. Molecular and cellular biology. 35(1):167-81. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"