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Product Name
Nucleostemin antibody
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Description
Nucleostemin Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in K-562 cells, HEK-293 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 60-62 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
C77032 antibody; E2 induced gene 3 protein antibody; E2IG3 antibody; GNL3 antibody; NNP47 antibody; Novel nucleolar protein 47 antibody; Nucleostemin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Nucleostemin recombinant protein (Accession Number: NM_014366). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113410(GNL3 antibody) at dilution of 1:1500
IP Result of anti-GNL3 (IP:Catalog No:113410, 4ug; Detection:Catalog No:113410 1:600) with HeLa cells lysate 4000ug.
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Background
GNL3 represents guanine nucleotide binding protein-like 3 (nucleolar)
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