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Product Name
nucleobindin 1 antibody
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Description
nucleobindin 1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, MCF7 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human spleen tissue, human kidney tissue, human liver tissue, human placenta tissue. Observed molecular weight by Western-blot: 63 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CALNUC antibody; DKFZp686A15286 antibody; FLJ40471 antibody; NUC antibody; NUCB1 antibody; nucleobindin 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of nucleobindin 1 recombinant protein (Accession Number: BC015787). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113407(NUCB1 antibody) at dilution of 1:500
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:113407(NUCB1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:113407(NUCB1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:113407(NUCB1 antibody) at dilution of 1:100 (under 40x lens)
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Background
Nucleobindin (Nuc) was first identified as a secreted protein of 55 kDa that promotes production of DNA-specific antibodies in lupus-prone MRL/lpr mice. Nuc contains a signal peptide, a DNA-binding site, two calcium-binding motifs (EF-hand motifs), and a leucine zipper. Nucleobindin is found in both cytosol and membrane and is localized to cis-Golgi cisternae and the cis-Golgi network (CGN). Nucleobindin is involved in autoimmunity, apoptosis and calcium homeostasis in the bone matrix. NUC was located at human chromosome 19q13.2-q13.4.
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