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NUCKS1 antibody

Cat.#: 113406

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Product Information

  • Product Name
    NUCKS1 antibody
  • Documents
  • Description
    NUCKS1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive WB detected in SGC-7901 cells, HEK-293 cells, L02 cells, MCF-7 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 45 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FLJ21480 antibody; FLJ32016 antibody; FLJ38536 antibody; JC7 antibody; NUCKS antibody; NUCKS1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NUCKS1 recombinant protein (Accession Number: NM_022731). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:113406(NUCKS1 antibody) at dilution of 1:400

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:113406(NUCKS1 antibody) at dilution of 1:400

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113406(NUCKS1 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113406(NUCKS1 antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-NUCKS1 (IP:Catalog No:113406, 4ug; Detection:Catalog No:113406 1:500) with HEK-293 cells lysate 2800ug.

    IP Result of anti-NUCKS1 (IP:Catalog No:113406, 4ug; Detection:Catalog No:113406 1:500) with HEK-293 cells lysate 2800ug.

  • Background
    Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1) is a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated by CDK1 and casein kinase during mitosis of the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Widelyexpressed, with highest levels in thyroid gland, prostate and uterus and in fetal liver, thymus and lung. Two isoforms of NUCKS1 exist due to alternative splicing events.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"