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Product Name
NUBP1 Polyclonal Antibody
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Description
Polyclonal antibody to NUBP1
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
NUBP1 antibody; NBP antibody; NBP1 antibody; NBP35 antibody; nucleotide binding protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 201-320 of human NUBP1 (NP_002475.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - NUBP1 Polyclonal Antibody
Western blot analysis of extracts of HepG2 cells, using NUBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - NUBP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using NUBP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NUBP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human thyroid cancer using NUBP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NUBP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using NUBP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NUBP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using NUBP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NUBP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using NUBP1 antibody at dilution of 1:100 (40x lens).
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Background
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication (By similarity). Negatively regulates cilium formation and structure (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"