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Product Name
NUB1 antibody
- Documents
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Description
NUB1 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HeLa cells, COLO 320 cells, HepG2 cells, K-562 cells, mouse kidney tissue, Raji cells. Observed molecular weight by Western-blot: 69 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BS4 antibody; NEDD8 ultimate buster 1 antibody; NUB1 antibody; NUB1L antibody; NYREN18 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NUB1 recombinant protein (Accession Number: NM_016118). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113403(NUB1 antibody) at dilution of 1:600
IP Result of anti-NUB1 (IP:Catalog No:113403, 3ug; Detection:Catalog No:113403 1:600) with HEK-293 cells lysate 2500ug.
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Background
Proteasome-associated protein NEDD8 ultimate buster 1 (NUB1) is an interferon-inducible protein that down-regulates NEDD8 expression and its conjugation system. Over-expression of NUB1 induces a growth-inhibitory effect in cells, and it mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Expression of NUB1 leads to decreased modification of p53 with NEDD8 and stimulation of p53 ubiquitination. Recent finding revealed that NUB1, by regulating GSK3β levels, modulates tau phosphorylation and aggregation, and is a key player in neurodegeneration associated with tau pathology.
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References
- Mori F, Watanabe Y, Miki Y. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology : official journal of the Japanese Society of Neuropathology. 34(2):140-7. 2014.
- Tanji K, Miki Y, Maruyama A. The role of NUB1 in α-synuclein degradation in Lewy body disease model mice. Biochemical and biophysical research communications. 470(3):635-42. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"