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Product Name
NTS Polyclonal Antibody
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Description
Polyclonal antibody to NTS
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NTS antibody; NMN-125 antibody; NN antibody; NT antibody; NT/N antibody; NTS1 antibody; neurotensin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-148 of human NTS (NP_006174.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - NTS Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NTS antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - NTS Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using NTS antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NTS Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using NTS antibody at dilution of 1:100 (40x lens).
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Background
Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"