-
Product Name
NTN1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to NTN1
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
NTN1 antibody; NTN1L antibody; netrin-1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human NTN1 (NP_004813.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - NTN1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines,using NTN1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Immunofluorescence - NTN1 Polyclonal Antibody
Immunofluorescence analysis of U-251MG cells using NTN1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"