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Product Name
NSE antibody
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Description
NSE Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, mouse brain tissue. Positive IHC detected in human testis tissue, human brain tissue, human lung tissue. Observed molecular weight by Western-blot: 47kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
enolase 2 (gamma antibody; neuronal) antibody; Gamma enolase antibody; Neural enolase antibody; Neuron specific enolase antibody; NSE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NSE recombinant protein (Accession Number: NM_001975). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113288(ENO2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human testis using Catalog No:113288(ENO2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:113288(ENO2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ENO2 (IP:Catalog No:113288, 4ug; Detection:Catalog No:113288 1:300) with mouse brain tissue lysate 4000ug.
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Background
Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), particularly isoform neuron-specific enolase (NSE), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors(PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.
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