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Product Name
NSBP1 antibody
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Description
NSBP1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive WB detected in PC-3 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
HMGN5 antibody; NSBP1 antibody; nucleosomal binding protein 1 antibody; Nucleosome binding protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NSBP1 recombinant protein (Accession Number: NM_030763). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113284(NSBP1 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:113284(NSBP1 Antibody) at dilution of 1:200 (under 40x lens)
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Background
NSBP1, also named Nucleosome-binding protein 1 or HMGN5, is a 282 amino acid protein, which belongs to the HMGN family. NSBP1 localizes in the nucleus and is ubiquitously expressed. NSBP1 preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. Western blots shows the expression of NSBP1 as a 55kDa protein in human bladder cancer cells. (PMID: 21695596).
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