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Product Name
NRD1 antibody
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Description
NRD1 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 135-140kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hNRD1 antibody; hNRD2 antibody; N arginine dibasic convertase antibody; Nardilysin antibody; NRD C antibody; NRD convertase antibody; NRD1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NRD1 recombinant protein (Accession Number: NM_001101662). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113275(NRD1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human testis using Catalog No:113275(NRD1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:113275(NRD1 antibody) at dilution of 1:50 (under 40x lens)
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Background
NRD1(Nardilysin) is also named as NRD-C(N-arginine dibasic convertase). It is a zinc metalloendopeptidase of the M16 pitrilysin family that cleaves peptides at dibasic residues. The enzyme has also been found in neural tissues of mouse embryos indicating a role in neural development. Although nardilysin is primarily found in the cytosol, it has been reported that nardilysin is located on the cell surface acting as a receptor for heparinbinding EGF-like growth factor(PMID:15809022). It has 2 isoforms produced by alternative splicing.
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References
- Radichev IA, Maneva-Radicheva LV, Amatya C. Nardilysin-dependent proteolysis of cell-associated VTCN1 (B7-H4) marks type 1 diabetes development. Diabetes. 63(10):3470-82. 2014.
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