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Product Name
NR3C2 antibody
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Description
NR3C2 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells. Positive IHC detected in human kidney tissue, human colon tissue, human skin tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 94-100kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MCR antibody; MineralOCorticoid receptor antibody; MLR antibody; NR3C2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NR3C2 recombinant protein (Accession Number: NM_001354819). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113268(NR3C2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113268(NR3C2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113268(NR3C2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using NR3C2 antibody Catalog No:113268 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NR3C2 (IP:Catalog No:113268, 4ug; Detection:Catalog No:113268 1:500) with HEK-293 cells lysate 3200ug.
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Background
Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.This antibody is a rabbit polyclonal antibody, sepcifically reacts with the 107kd MCR protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"