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Product Name
NR2B antibody
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Description
NR2B Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive IF detected in SH-SY5Y cells. Positive WB detected in mouse brain tissue, human brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 166 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GluN2B antibody; GRIN2B antibody; hNR3 antibody; NMDAR2B antibody; NR2B antibody; NR3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NR2B recombinant protein (Accession Number: XM_017019219). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113235(GRIN2B antibody) at dilution of 1:5000
IP Result of anti-GRIN2B (IP:Catalog No:113235, 3ug; Detection:Catalog No:113235 1:2000) with mouse brain tissue lysate 6000ug.
Immunohistochemical of paraffin-embedded human brain using Catalog No:113235(GRIN2B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113235(GRIN2B antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:113235(GRIN2B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GRIN2B represents glutamate receptor, ionotropic, N-methyl D-aspartate 2B
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References
- Liu XS, Hou Y, Yan TL. Dopamine D3 receptor-regulated NR2B subunits of N-methyl-d-aspartate receptors in the nucleus accumbens involves in morphine-induced locomotor activity. CNS neuroscience & therapeutics. 20(9):823-9. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"