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Product Name
NR1I3 Polyclonal Antibody
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Description
Polyclonal antibody to NR1I3
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NR1I3 antibody; CAR antibody; CAR1 antibody; MB67 antibody; nuclear receptor subfamily 1 group I member 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 103-352 of human NR1I3 (NP_001070948.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - NR1I3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NR1I3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - NR1I3 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NR1I3 antibody . Blue: DAPI for nuclear staining.
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Background
Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"