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Product Name
NPR3 Polyclonal Antibody
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Description
Polyclonal antibody to NPR3
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NPR3 antibody; ANP-C antibody; ANPR-C antibody; ANPRC antibody; C5orf23 antibody; GUCY2B antibody; NPR-C antibody; NPRC antibody; natriuretic peptide receptor 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 272-541 of human NPR3 (NP_001191304.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - NPR3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NPR3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.
Immunofluorescence - NPR3 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NPR3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"