NPHS1 Polyclonal Antibody

Cat.#: 163994

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Product Information

  • Product Name
    NPHS1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to NPHS1
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse
  • Alternative names
    NPHS1 antibody; CNF antibody; NPHN antibody; nephrin antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human NPHS1
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:200
  • Validations

    Western blot - NPHS1 Polyclonal Antibody

    Western blot - NPHS1 Polyclonal Antibody

    Western blot analysis of extracts of mouse kidney, using NPHS1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.

    Western blot - NPHS1 Polyclonal Antibody

    Western blot - NPHS1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using NPHS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunohistochemistry - NPHS1 Polyclonal Antibody

    Immunohistochemistry - NPHS1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse kidney using NPHS1 antibody (40x lens).

    Immunofluorescence - NPHS1 Polyclonal Antibody

    Immunofluorescence - NPHS1 Polyclonal Antibody

    Immunofluorescence analysis of mouse kidney cells using NPHS1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - NPHS1 Polyclonal Antibody

    Immunofluorescence - NPHS1 Polyclonal Antibody

    Immunofluorescence analysis of mouse kidney cells using NPHS1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"