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Product Name
NPHS1 Polyclonal Antibody
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Description
Polyclonal antibody to NPHS1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
NPHS1 antibody; CNF antibody; NPHN antibody; nephrin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human NPHS1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200 -
Validations
Western blot - NPHS1 Polyclonal Antibody
Western blot analysis of extracts of mouse kidney, using NPHS1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Western blot - NPHS1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NPHS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - NPHS1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using NPHS1 antibody (40x lens).
Immunofluorescence - NPHS1 Polyclonal Antibody
Immunofluorescence analysis of mouse kidney cells using NPHS1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - NPHS1 Polyclonal Antibody
Immunofluorescence analysis of mouse kidney cells using NPHS1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"