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Product Name
NPHP4 antibody
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Description
NPHP4 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue. Positive IF detected in hTERT-RPE1 cells. Positive WB detected in mouse brain tissue, Y79 cells. Observed molecular weight by Western-blot: 150 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
KIAA0673 antibody; NephrOCystin 4 antibody; nephronophthisis 4 antibody; Nephroretinin antibody; NPHP4 antibody; SLSN4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NPHP4 recombinant protein (Accession Number: BC040520). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113214(NPHP4 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human heart using Catalog No:113214(NPHP4 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:113214(NPHP4 antibody) at dilution of 1:50 (under 40x lens)
IF result from Dr. Corbit, Kevin. anti-NPHP4(Catalog No:113214) marks the ciliary axoneme, basal bodies, and the transition zone of Human hTERT-RPE1 cells.
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Background
NPHP4, also named as KIAA0673, is a cilia-associated protein which involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8. It is necessary for normal photoreceptor ribbon synapse maintenance and outer segment formation, and for sperm development.
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References
- Garcia-Gonzalo FR, Corbit KC, Sirerol-Piquer MS. A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. Nature genetics. 43(8):776-84. 2011.
- Bangs FK, Schrode N, Hadjantonakis AK, Anderson KV. Lineage specificity of primary cilia in the mouse embryo. Nature cell biology. 17(2):113-22. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"