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Product Name
NPHP3 antibody
- Documents
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Description
NPHP3 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue. Positive IF detected in hTERT-RPE1 cells. Positive IP detected in mouse testis tissue. Positive WB detected in human testis tissue, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse testis tissue. Observed molecular weight by Western-blot: 145-151 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DKFZp667K242 antibody; DKFZp781K1312 antibody; FLJ30691 antibody; FLJ36696 antibody; KIAA2000 antibody; MKS7 antibody; NephrOCystin 3 antibody; NPHP3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NPHP3 recombinant protein (Accession Number: BC068082). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
IF result (the base and the lower half of cilia stain) of anti-NPHP3 (Catalog No:113213, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113213(NPHP3 antibody) at dilution of 1:500
IP Result of anti-NPHP3 (IP:Catalog No:113213, 4ug; Detection:Catalog No:113213 1:1000) with mouse testis tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:113213(NPHP3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:113213(NPHP3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
NPHP3 represents nephronophthisis 3 (adolescent)
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References
- Su S, Phua SC, DeRose R. Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. Nature methods. 10(11):1105-7. 2013.
- Schwarz N, Carr AJ, Lane A. Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelium cells. Human molecular genetics. 24(4):972-86. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"