-
Product Name
NOXO1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to NOXO1
-
Tested applications
WB
-
Species reactivity
Mouse
-
Alternative names
NOXO1 antibody; P41NOX antibody; P41NOXA antibody; P41NOXB antibody; P41NOXC antibody; SH3PXD5 antibody; SNX28 antibody; NADPH oxidase organizer 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 121-370 of human NOXO1 (NP_653204.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - NOXO1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NOXO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
-
Background
Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"