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Product Name
NOX2 antibody
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Description
NOX2 Rabbit Polyclonal antibody. Positive IHC detected in human skin tissue. Positive WB detected in mouse brain tissue, human serum tissue, mouse heart tissue, Raji cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CYBB antibody; CytOChrome b 245 heavy chain antibody; NOX2 antibody; p91 PHOX antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NOX2 recombinant protein (Accession Number: NM_000397). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113312(CYBB antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:113312(CYBB Antibody) at dilution of 1:200 (under 40x lens).
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Background
NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781). In our results, it reavels the bands of 55-65 kDa and 80 kDa besides a band of 140 kDa that we do not find the reason.
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References
- Wang J, Shao M, Liu M. PKCα promotes generation of reactive oxygen species via DUOX2 in hepatocellular carcinoma. Biochemical and biophysical research communications. 463(4):839-45. 2015.
- Zhang Y, Wei X, Liu L. TIPE2, a novel regulator of immunity, protects against experimental stroke. The Journal of biological chemistry. 287(39):32546-55. 2012.
- Fan YF, Zhang R, Jiang X. The phosphodiesterase-5 inhibitor vardenafil reduces oxidative stress while reversing pulmonary arterial hypertension. Cardiovascular research. 99(3):395-403. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"