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Product Name
NOS1 Polyclonal Antibody
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Description
Polyclonal antibody to NOS1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NOS1 antibody; IHPS1 antibody; N-NOS antibody; NC-NOS antibody; NOS antibody; bNOS antibody; nNOS antibody; nitric oxide synthase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human NOS1 (NP_000611.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:20 - 1:50 -
Validations
Western blot - NOS1 Polyclonal Antibody
Western blot analysis of extracts of Rat brain, using NOS1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - NOS1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using NOS1 antibody . Blue: DAPI for nuclear staining.
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Background
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"