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Product Name
NONO antibody
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Description
NONO Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive FC detected in HepG2 cells. Positive IP detected in HEK-293 cells. Positive WB detected in MCF7 cells, HEK-293 cells, human pancreas tissue, human skeletal muscle tissue, mouse pancreas tissue. Observed molecular weight by Western-blot: 54kd
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
55 kDa nuclear protein antibody; NONO antibody; NonO protein antibody; NRB54 antibody; P54 antibody; p54(nrb) antibody; P54NRB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NONO recombinant protein (Accession Number: BC010049). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113302(NONO antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113302(NONO antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using NONO antibody Catalog No:113302 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HepG2 cells were stained with 0.2ug NONO antibody (Catalog No:113302, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP Result of anti-NONO (IP:Catalog No:113302, 4ug; Detection:Catalog No:113302 1:1000) with HEK-293 cells lysate 2800ug.
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Background
Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.
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References
- Yang X, Zou P, Yao J. Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma. Journal of proteome research. 9(3):1402-15. 2010.
- He YF, Bao HM, Xiao XF. Biotin tagging coupled with amino acid-coded mass tagging for efficient and precise screening of interaction proteome in mammalian cells. Proteomics. 9(24):5414-24. 2009.
- Tang S, Bao H, Zhang Y, Yao J, Yang P, Chen X. 14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage. PloS one. 8(3):e55268. 2013.
- Yadav SP, Hao H, Yang HJ. The transcription-splicing protein NonO/p54nrb and three NonO-interacting proteins bind to distal enhancer region and augment rhodopsin expression. Human molecular genetics. 23(8):2132-44. 2014.
- Park M, Lim JS, Lee HJ. Distinct Protein Expression Profiles of Solid-Pseudopapillary Neoplasms of the Pancreas. Journal of proteome research. 14(8):3007-14. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"