-
Product Name
NOL3 antibody
- Documents
-
Description
NOL3 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse skeletal muscle tissue, HeLa cells. Observed molecular weight by Western-blot: 30-33kd
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
Apoptosis repressor with CARD antibody; ARC antibody; CARD2 antibody; MYC antibody; MYP antibody; NOL3 antibody; NOP antibody; NOP30 antibody; Nucleolar protein 3 antibody; Nucleolar protein of 30 kDa antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of NOL3 recombinant protein (Accession Number: NM_001276312). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:113293(NOL3 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113293(NOL3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using NOL3 antibody Catalog No:113293 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
-
Background
NOL3 represents nucleolar protein 3 (apoptosis repressor with CARD domain)
-
References
- Li X, Du N, Zhang Q. MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy. Cell death & disease. 5:e1479. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"