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Product Name
NOL12 antibody
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Description
NOL12 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dJ37E16.7 antibody; FLJ34609 antibody; NOL12 antibody; Nop25 antibody; nucleolar protein 12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NOL12 recombinant protein (Accession Number: NM_024313). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113292(NOL12 antibody) at dilution of 1:1000
IP Result of anti-NOL12 (IP:Catalog No:113292, 4ug; Detection:Catalog No:113292 1:300) with HeLa cells lysate 4000ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:113292(NOL12 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NOL12 represents nucleolar protein 12
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References
- Jäger S, Cimermancic P, Gulbahce N. Global landscape of HIV-human protein complexes. Nature. 481(7381):365-70. 2012.
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