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Product Name
NOB1 antibody
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Description
NOB1 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue, human colon cancer tissue. Positive IP detected in HepG2 cells. Positive WB detected in SGC-7901 cells. Observed molecular weight by Western-blot: 47-50 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ART 4 antibody; ART4 antibody; MST158 antibody; MSTP158 antibody; NOB1 antibody; NOB1P antibody; Protein ART 4 antibody; PSMD8BP1 antibody; RNA binding protein NOB1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NOB1 recombinant protein (Accession Number: BC000050). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:10-1:100
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Validations
SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:113262(NOB1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:113262(NOB1 antibody) at dilution of 1:25 (under 10x lens)
IP Result of anti-NOB1 (IP:Catalog No:113262, 4ug; Detection:Catalog No:113262 1:300) with HepG2 cells lysate 2400ug.
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Background
NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.
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References
- He XW, Feng T, Yin QL, Jian YW, Liu T. NOB1 is essential for the survival of RKO colorectal cancer cells. World journal of gastroenterology. 21(3):868-77. 2015.
- Schütz S, Fischer U, Altvater M. A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly. eLife. 3:e03473. 2014.
- Wu DP, He XW.
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