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Product Name
NO66/C14orf169 antibody
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Description
NO66/C14orf169 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in A431 cells, Jurkat cells. Observed molecular weight by Western-blot: 71 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
C14orf169 antibody; FLJ21802 antibody; hsNO66 antibody; MAPJD antibody; NO66 antibody; Nucleolar protein 66 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NO66/C14orf169 recombinant protein (Accession Number: NM_024644). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:113261(C14orf169 Antibody) at dilution of 1:600
IP Result of anti-C14orf169 (IP:Catalog No:113261, 4ug; Detection:Catalog No:113261 1:500) with Jurkat cells lysate 4000ug.
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Background
NO66, also known as C14orf169 and MAPJD, is a JmjC domain-containing oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. NO66 is highly conserved during evolution and shows a dual localization pattern, present in both the nucleoplasm and nucleolus (PMID: 14742713). It has been reported that NO66 participates in regulation of Osx transcriptional activity and plays an important role in osteoblast differentiation through interaction with Osx (PMID: 19927124; 23620590). Through interaction with MYC protein, NO66 plays a role in pulmonary carcinogenesis (PMID: 17308053).
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