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Product Name
NME1 antibody
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Description
NME1 Rabbit Polyclonal antibody. Positive IP detected in A549 cells. Positive WB detected in HeLa cells, A549 cells, MCF7 cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human lung cancer tissue, human pancreas cancer tissue. Observed molecular weight by Western-blot: 16-20 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AWD antibody; GAAD antibody; Granzyme A activated DNase antibody; NBS antibody; NDK A antibody; NDP kinase A antibody; NDPK A antibody; NDPKA antibody; NME1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NME1 recombinant protein (Accession Number: NM_000269). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113246(NME1 antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113246(NME1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113246(NME1 antibody) at dilution of 1:100 (under 40x lens)
WB result of Catalog No:(NME1 antibody).
IP Result of anti-NME1 (IP:Catalog No:113246, 4ug; Detection:Catalog No:113246 1:500) with A549 cells lysate 2800ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:113246(NME1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination.It has 3 isoforms produced by alternative splicing.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"