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Product Name
NMD3 antibody
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Description
NMD3 Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 07 antibody; FLJ21053 antibody; hNMD3 antibody; NMD3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NMD3 recombinant protein (Accession Number: NM_015938). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113244(NMD3 antibody) at dilution of 1:1000
IP Result of anti-NMD3 (IP:Catalog No:113244, 3ug; Detection:Catalog No:113244 1:800) with HepG2 cells lysate 1720ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:113244(NMD3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Human NMD3 gene encodes 60S ribosomal export protein which was found in a 60S ribosomal subunit export complex with RAN and XPO1. This nucleocytoplasmic shuttling protein NMD3 is an adaptor for export of the 60S ribosomal subunit from the nucleus. NMD3 contains a CRM-1-dependent leucine-rich nuclear export signal (NES) and a dispersed nuclear localization signal (NLS), the basic region of which is also required for nucleolar accumulation. NMD3 are required for nuclear export of the 60S ribosomal subunit in yeast and vertebrate cells, recent finding has also revealed its role in Arabidopsis thaliana.
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References
- Wong CC, Traynor D, Basse N, Kay RR, Warren AJ. Defective ribosome assembly in Shwachman-Diamond syndrome. Blood. 118(16):4305-12. 2011.
- Finch AJ, Hilcenko C, Basse N. Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome. Genes & development. 25(9):917-29. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"