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Product Name
NLRP7 Polyclonal Antibody
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Description
Polyclonal antibody to NLRP7
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NLRP7 antibody; CLR19.4 antibody; HYDM antibody; NALP7 antibody; NOD12 antibody; PAN7 antibody; PYPAF3 antibody; NLR family pyrin domain containing 7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 800-1000 of human NLRP7 (NP_631915.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - NLRP7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NLRP7 Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.
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Background
Inhibits CASP1/caspase-1-dependent IL1B secretion.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"