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Product Name
NLRP2 Polyclonal Antibody
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Description
Polyclonal antibody to NLRP2
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
NLRP2 antibody; CLR19.9 antibody; NALP2 antibody; NBS1 antibody; PAN1 antibody; PYPAF2 antibody; NLR family pyrin domain containing 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NLRP2 (NP_060322.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - NLRP2 Polyclonal Antibody
Western blot analysis of extracts of A-549 cells, using NLRP2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - NLRP2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NLRP2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"