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Product Name
NKX2-5 Polyclonal Antibody
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Description
Polyclonal antibody to NKX2-5
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NKX2-5 antibody; CHNG5 antibody; CSX antibody; CSX1 antibody; HLHS2 antibody; NKX2.5 antibody; NKX2E antibody; NKX4-1 antibody; VSD3 antibody; NK2 homeobox 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NKX2-5 (NP_004378.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - NKX2-5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NKX2-5 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunofluorescence - NKX2-5 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NKX2-5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). Binds to the core DNA motif of NPPA promoter. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
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