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  • NIN Polyclonal Antibody
  • NIN Polyclonal Antibody

NIN Polyclonal Antibody

Cat.#: 161233

Special Price 174.2 USD

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Product Information

  • Product Name
    NIN Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to NIN
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    NIN antibody; SCKL7 antibody; ninein antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-665 of human NIN (NP_891991.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - NIN Polyclonal Antibody

    Western blot - NIN Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using NIN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunofluorescence - NIN Polyclonal Antibody

    Immunofluorescence - NIN Polyclonal Antibody

    Immunofluorescence analysis of U2OS cells using NIN antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Required for centriole organization and microtubule anchoring at the mother centriole.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"