Nigrosin-OG2 peptide

Nigrosin-OG2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-GLLGKILGVEKKVLCGLSGMC-OH

H-Gly-Leu-Leu-Gly-Lys-Ile-Leu-Gly-Val-Glu-Lys-Lys-Val-Leu-Cys-Gly-Leu-Ser-Gly-Met-Cys-OH

21

95.39%

C₉₃H₁₆₈N₂₄O₂₅S₃

2118.66

Synthetic

Powder

Nigrosin-OG2 is a peptide that has been studied for its antimicrobial and hemolytic activities. It is a member of the nigrocin-2 family of peptides, which are found in the skin secretions of the Yunnanfu frog (Rana grahami / Huia grahami).
Studies have shown that Nigrosin-OG2 exhibits reduced hemolytic activity against human erythrocytes compared to other peptides in the same family. This decrease in hemolytic activity is likely due to the replacement of polar residues on the hydrophilic face of the peptide's alpha-helix with neutral amino acids, resulting in a decrease in amphipathicity.
The primary structure of Nigrosin-OG2 has been determined and it has been found to be identical to two other peptides, grahamin 1 and grahamin 2, which were also isolated from R. grahami skin secretions.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Li J, Xu X, Xu C, et al. Anti-infection peptidomics of amphibian skin. Mol Cell Proteomics. 2007;6(5):882-94.
• Conlon JM, Al-Ghaferi N, Abraham B, Jiansheng H, Cosette P, Leprince J, Jouenne T, Vaudry H. Antimicrobial peptides from diverse families isolated from the skin of the Asian frog, Rana grahami. Peptides. 2006 Sep;27(9):2111-7.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.