Nigrocin-2 peptide
Not For Human Use, Lab Use Only.
Cat.#: 309452
Optional Service:
TFA RemovalWhat's this?
Special Price 375.7 USD
Availability:
3 weeks
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Product Name
Nigrocin-2 peptide
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Sequence Shortening
GLLSKVLGVGKKVLCGVSGLC
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Sequence
H-Gly-Leu-Leu-Ser-Lys-Val-Leu-Gly-Val-Gly-Lys-Lys-Val-Leu-Cys-Gly-Val-Ser-Gly-Leu-Cys-OH (disulfide bond: Cys15-Cys21)
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Length (aa)
21
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Peptide Purity (HPLC)
96.5%
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Molecular Formula
C90H162N24O24S2
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Molecular Weight
2028.5
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Source
Synthetic
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Form
Powder
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Description
Note that nigrocin-2LVa, nigrocin-2 (or nigrocin-2N), and nigrocin-2HJ share the same primary structure. Active against M. luteus (MIC 2.5 ug/ml), Gram-negative S. dysenteriae, K. pneumoniae (MIC 10 ug/ml), P. aeruginosa (MIC 100 ug/ml), and S. typhimurium (MIC 22 ug/ml), and weakly active against C. albicans (MIC 150 ug/ml). Nigrocin 2 consists of an amphipathic alpha-helix from residue 3-18 in 50% TFE or SDS micelles. Updated 7/2013. Nigrocin-2 has Antimicrobial activity. The source of Nigrocin-2 is Pelophylax nigromaculatus (OLD Rana nigromaculata), Asia.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Park S, Park SH, Ahn HC, et al. Structural study of novel antimicrobial peptides, nigrocins, isolated from Rana nigromaculata. FEBS Lett. 2001;507(1):95-100.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Peptide Property
- Analysed Sequence:H-GLLSKVLGVGKKVLCGVSGLC-OH
- Chemical Formula:C90H162N24O24S2
- Sequence length:21
- Extinction coefficient:120 M-1cm-1
- GRAVY:1.21
- Mw average:2028.52
- Theoretical pI:9.8
- Data Source:Peptide Property Calculator
GRAVY = grand average of hydropathy
X: Hydrophobic uncharged residues, like F I L M V W A and P
X: Basic residues, like R K H
X: Acidic residues, like D E
X: Polar uncharged residues, like G S T C N Q and Y
Related Products / Services
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• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"