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Product Name
Human PBEF / Visfatin / NAMPT (His & GST Tag) recombinant protein
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Description
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
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Protein name
Nicotinamide phosphoribosyltransferase
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Protein short names
PBEF; NAMPRTASE; VISFATIN; 1110035O14RIK; VF; PBEF1; AI314458; DKFZP666B131; AI480535; MGC117256
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Uniprot ID
P43490
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Gene Name
NAMPT; PBEF; PBEF1
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human NAMPT (P43490) (Met 1-His 491 was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human NAMPT/GST chimera consists of 728 amino acids and has a calculated molecular mass of 83.3 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Validations
Human PBEF / Visfatin / NAMPT Protein (His & GST Tag) SDS-PAGE
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