-
Product Name
Anti-PBEF / Visfatin / NAMPT antibody
- Documents
-
Description
Rabbit polyclonal to PBEF / Visfatin / NAMPT
-
Tested applications
ELISA, WB, IHC-P, IP
-
Species reactivity
Human NAMPT / PBEF
-
Alternative names
Pbef antibody; PBEF1 antibody; VF antibody; Visfatin antibody; 1110035O14Rik antibody; 1110035O14Rik antibody; AI314458 antibody; AI480535 antibody; DKFZp666B131 antibody; MGC117256 antibody; NAmPRTase antibody; PBEF antibody; Pbef1 antibody; VISFATIN antibody; VF antibody; PBEF antibody; PBEF1 antibody; VISFATIN antibody; 1110035O14Rik antibody; Pbef antibody; Pbef1 antibody; AI314458 antibody; AI480535 antibody; Visfatin antibody; NAmPRTase antibody; 1110035O14Rik antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Human NAMPT / PBEF (rh NAMPT / PBEF; P43490; Met1-His491). NAMPT / PBEF specific IgG was purified by Human NAMPT / PBEF affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human NAMPT / PBEF. The detection limit for Human NAMPT / PBEF is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
-
Validations
PBEF / Visfatin / NAMPT Antibody, Rabbit PAb, Antigen Affinity Purified
PBEF / Visfatin / NAMPT Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human NAMPT in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
PBEF / Visfatin / NAMPT Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human NAMPT in human liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
PBEF / Visfatin / NAMPT Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
-
Background
Nicotinamide phosphoribosyltransferase (NAMPT), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme belonging to the family of glycosyltransferases, to be specific, the pentosyltransferases. This enzyme participates in nicotinate and nicotinamide metabolism. This enzyme catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1- pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. NAMPT is also considered as an essential enzyme mediating granulocyte colony-stimulating factor (G-CSF)-triggered granulopoiesis in healthy individuals and in individuals with severe congenital neutropenia. Intracellular NAMPT and NAD+ amounts in myeloid cells, as well as plasma NAMPT and NAD+ levels, were increased by G-CSF treatment of both healthy volunteers and individuals with congenital neutropenia.
-
References
- Skokowa J, et al. (2009) NAMPT is essential for the G-CSF-induced myeloid differentiation via a NAD+-sirtuin-1-dependent pathway. Nat Med. 15(2): 151-8.
- Samal B, et al. (1994) Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor. Mol Cell Biol. 14 (2): 1431-7.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"