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Product Name
NGFRAP1 Polyclonal Antibody
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Description
Polyclonal antibody to NGFRAP1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
BEX3 antibody; Bex antibody; DXS6984E antibody; HGR74 antibody; NADE antibody; NGFRAP1 antibody; protein BEX3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-111 of human NGFRAP1 (NP_996798.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - NGFRAP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NGFRAP1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunofluorescence - NGFRAP1 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using NGFRAP1 antibody .
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Background
May be a signaling adapter molecule involved in p75NTR-mediated apoptosis induced by NGF. Plays a role in zinc-triggered neuronal death (By similarity). May play an important role in the pathogenesis of neurogenetic diseases.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"