-
Product Name
NFYC Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to NFYC
-
Tested applications
WB
-
Species reactivity
Mouse
-
Alternative names
NFYC antibody; CBF-C antibody; CBFC antibody; H1TF2A antibody; HAP5 antibody; HSM antibody; NF-YC antibody; nuclear transcription factor Y subunit gamma antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 86-335 of human NFYC (NP_055038.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - NFYC Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NFYC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"