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Product Name
NFYB Polyclonal Antibody
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Description
Polyclonal antibody to NFYB
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
NFYB antibody; CBF-A antibody; CBF-B antibody; HAP3 antibody; NF-YB antibody; nuclear transcription factor Y subunit beta antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human NFYB (NP_006157.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - NFYB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NFYB antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - NFYB Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using NFYB antibody . Blue: DAPI for nuclear staining.
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Background
Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"