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Product Name
NFU1 Polyclonal Antibody
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Description
Polyclonal antibody to NFU1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NFU1 antibody; CGI-33 antibody; HIRIP antibody; HIRIP5 antibody; MMDS1 antibody; NIFUC antibody; Nfu antibody; NifU antibody; NFU1 iron-sulfur cluster scaffold antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human NFU1 (NP_001002755.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - NFU1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NFU1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Western blot - NFU1 Polyclonal Antibody
Western blot analysis of extracts of various cells, using NFU1 antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Immunohistochemistry - NFU1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse intestine using NFU1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NFU1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using NFU1 Antibody at dilution of 1:100 (40x lens).
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Background
Iron-sulfur cluster scaffold protein which can assemble [4Fe-4S] clusters and deliver them to target proteins.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"