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Product Name
NFU1 antibody
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Description
NFU1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in mouse brain tissue. Positive WB detected in mouse cerebellum tissue, mouse brain tissue, mouse testis tissue. Observed molecular weight by Western-blot: 23-28 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CGI 33 antibody; HIRA interacting protein 5 antibody; HIRIP5 antibody; Nfu antibody; NFU1 antibody; NifU antibody; NIFUC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NFU1 recombinant protein (Accession Number: NM_001002755). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with Catalog No:113165(NFU1 Antibody) at dilution of 1:1000
IP Result of anti-NFU1 (IP:Catalog No:113165, 4ug; Detection:Catalog No:113165 1:1000) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:113165(NFU1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NFU1, also named as HIRA-interacting protein 5 or CGI-33, is a 254 amino acid protein, which belongs to the NifU family. NFU1 localizes in the Mitochondrion. NFU1 is widely expressed in adult tissues. NFU1, as an Iron-sulfur cluster scaffold protein, which can assemble clusters and deliver them to target proteins. It has an association with Multiple mitochondrial dysfunctions syndrome 1.
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