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Product Name
NFS1 antibody
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Description
NFS1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human heart tissue, human brain tissue, human kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue. Positive IP detected in HepG2 cells. Positive WB detected in A549 cells, HeLa cells, HepG2 cells, human heart tissue. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HUSSY 08 antibody; IscS antibody; NFS1 antibody; NIFS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NFS1 recombinant protein (Accession Number: NM_181679). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:113164(NFS1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:113164(NFS1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:113164(NFS1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using NFS1 antibody Catalog No:113164 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NFS1 (IP:Catalog No:113164, 4ug; Detection:Catalog No:113164 1:500) with HepG2 cells lysate 3200ug.
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Background
NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.
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