NFKBIZ Polyclonal Antibody
Polyclonal antibody to NFKBIZ
Human, Mouse, Rat
NFKBIZ antibody; IKBZ antibody; INAP antibody; MAIL antibody; NFKB inhibitor zeta antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NFKBIZ (NP_113607.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot - NFKBIZ Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NFKBIZ antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - NFKBIZ Polyclonal Antibody
Immunofluorescence analysis of Raw264.7 cells using NFKBIZ antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF-kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. May promote apoptosis (By similarity). Involved in the induction of T helper 17 cells (Th17) differentiation upon recognition of antigen by T cell antigen receptor (TCR) (By similarity).
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