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Product Name
NFIB Polyclonal Antibody
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Description
Polyclonal antibody to NFIB
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Tested applications
WB
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Species reactivity
Human
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Alternative names
NFIB antibody; CTF antibody; HMGIC/NFIB antibody; NF-I/B antibody; NF1-B antibody; NFI-B antibody; NFI-RED antibody; NFIB2 antibody; NFIB3 antibody; nuclear factor I B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human NFIB (NP_005587.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
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Validations
Western blot - NFIB Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using NFIB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 120s.
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Background
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"