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Product Name
NFIA antibody
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Description
NFIA Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in A431 cells, HeLa cells, Jurkat cells, L02 cells, mouse liver tissue. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 60-70kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CTF antibody; DKFZp434L0422 antibody; DKFZp686J23256 antibody; FLJ39164 antibody; KIAA1439 antibody; NF I/A antibody; NF1 A antibody; NFI A antibody; NFI L antibody; NFIA antibody; Nuclear factor 1 A type antibody; Nuclear factor 1/A antibody; nuclear factor I/A antibody; TGGCA binding protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NFIA recombinant protein (Accession Number: NM_005595). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:113150(NFIA antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113150(NFIA antibody) at dilution of 1:25 (under 10x lens)
IP Result of anti-NFIA (IP:Catalog No:113150, 4ug; Detection:Catalog No:113150 1:500) with A431 cells lysate 2000ug.
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Background
The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. Catalog# 11750-1-AP is a rabbit polyclonal antibody raised against N-terminal of human original NFIA.
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